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    1. “ϵ҂ | \ | Wվ؈D uXѪܲtԺ“ϵʽ XѪܲ\ uXѪܲtԺWվ؈D

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      ַؑctƴWٵһtԺ񽛃ȿƣ400016 ؑctƴW񽛲Wо400016 ժ Ҫ׼øCProtein Kinase C,PKCһɶNͬøMɵĽz/Kᵰ׼øPKC̎֬xĭhcֳֻ񽛂_{[lȲ^е“ϵоPKCȱѪXplC^ҪĔMPKCͬøśrcXȱѪPϵоFһC PI~׼øC ͬø XȱѪ PKC类_Jĵ׼ø֮һ1977NishizukaȌXMеĵ׼ø_JPKClFнM׼øܱԵˮøSоCPKC񽛼ܵ{^PI 1PKCW 1.1 PKCͬøķֲ[12] PKCһ}֬هữøVڄ΢ЙCwֲڃеĽMֹͬøĽYøIJͬȌ֞ĴʮNPDCͣcPKCsclassical PKC:I¢nRKCs(novel PKC):/aPKCs(atypical PKC):/PRKs(PKC-Related-Kinase):PRK1PRK2 PKCֲOVNͬøķֲָXMѰlF7NͬøPKCÞXͼPKCPKCPKCƵXMкdzS 1.2 PKCͬøĻY[15] PKCһl朶PKCͬøcDNAԓ朰О{regulatory domainȻО߻catalytic domain֮gͨ^ɱøˮġq朅^hinge domain,V3^Bԓ^øļ^аlĶʹPKCԲλ¶{C1C2/V0V1V2Ă^ø߻C3C4V4V5Ă^C1İ߀мٵλcpseudosubstrate sitePRKso˅^PKCڰ߀leaderͿĤtrans-membrane,TMɶPKCͬøV1^ĽYMɵIJQ˸ͬøĶ 1.3 PKC{down-regulation[23] ڼٵĴoϢBµPKCԟoʽڼ{̼PKCλtranslocationĤ̕PKC̖ͨ·µĶ¼xӃڵȣҪmPKCڼֳֻ[lȷҪmPKCɌ¼ʧ{PKC{ĬFQ顰PKC{PKC{cPPKCøԽ⣺PKCCa2+Եøcalpainڑጷź/򼤻ԴPKCƄష񽛹ȣ܅cȱѪlPKCԵ{̼̖øʹĤ֬YMְl׃ӰPKCĻܽʵӿPKC{XѪTPKCS½cP 2 PKCͬøcȱѪXpPϵ ȱѪXp^ѪҺДlһϵеļȴxKԪ׃Ȼ@һxPIhв˂lFPKC^Ҫ 2.1 XȱѪPKCԵ׃ о̕]̥PѪ܌̥XPKC{Ӱ푕rýMữPMAPKCYϷlFPKCλ]^LrgrPKC{[6]ã3HPKBu@Ӱg^̕ȱѪ20minٹrPKC׃ҎlFRCA1^ٹ612h_ٹ7d½[7]ȻHara[8]ھ˨MCAOȱѪ/ٹSV-129ģlFٹ1h[3H]PDBu@pٵȵ4050%37d½սM20%WҲƈЌW[9]FYIJڣȱѪ̼PKCλܰlȱѪFPKCԵ^LrgmȱѪ̼PKC{CƵącFPKCԵ^LrgmȱѪ̼PKC{CƵącPKC½ȻҲczyNټģ͵PYʾPKCcȱѪXpİllչ؄eǶ̕XȱѪlPKCcȱѪtlԓphλȱѪԓp[10] 2.2 ȱѪXMPKCͬøı_{ PKCcȱѪTȰd^TԪ׃PBuchner[11]ùȰPMA^^BRԪPKCͬøٷֲYlFHPKCÌȰББ_ÁxgͬøԿwWestern blot⹲۽R߼W^쵽ȰPMAɷNͬøٷֲвͬãPMAƒĤƬλȰ𼚰}Ķƒ򼚰Ǽλ߼WʾȰPKCҪ{ȼǼܘӽYλPKCٷֲĤM뼚߿ܽʾPKCd^ԓpҪIslam[12]^ɹɳȫXȱѪPKCõķֲlFȱѪ3hƤ|ȳFPKCԪ612h_߷7dܙzyPKCrںRƤ|@N׃ҎɿcȫXȱѪ{ͻ|ЧPWestem bolt߽Mg^칷ȫXȱѪҲlFȱѪԪpİlSPKCԵӶRCA1CA4СXPKCױ_PKCPKCßo׃fPKCcȱѪԪp^Ҿͬøخ[13] Savithiry[14]ɳ̕ȱѪٹעģȱѪ10minٹע\cDNAsgzyǰXPKCͬøƵmRNA_ٹע1624hNͬømRNA_Ԧ@3d֏ˮƽm7dMiettinen[15]\ԭλsgӡEimmunoblottingg^쾀˨MCAOȱѪٹWistarPKCmRNAsױ_׃ҎɣڹȱѪ30minٹ4hͬømRNAˮƽٹ12hȫʧڹ߅^ٹ41224hHPKC mRNAˮƽٹ37d܇^ischemic penumbraȱѪӰ^PKC mRNAˮƽҲٹע䣨2dȱѪ܇^PKCĵױ_ԪĿMK-801PKC mRNAˮƽױ_PKCĿͨ^{NMDAwȱѪXoW\RT-PCRgͬӵĄģͣȱѪ5min^쵽cPKCsЃHPKC mRNAˮƽٹڣ3hȣ_յ145%S 6065%3dصˮƽYIJȱѪrgͬ[16]M@ЩYPKCPKCÿȱѪXpҪ 2.3 PKCƄȱѪXpıo XMкSPKCͻ|ǰĩˣԪϢff|ጷŵȷҪmȻĿǰȱPKCͬøخƄһЩˎ_ʼ̽ӑPKCXȱѪCеѪܽYĴȱѪٹģп^쵽PKCwƬжڰ{ƬѸPKCˮƽ@\ùȰ׿ʹˮƽcȱѪٹйȰጷŵĜpP[17]wؿԜpȱѪTPKCλڻpȱѪTPKCλpȱѪTf|ጷȱѪ^PKCӄPDBu׿staruosporineڻȱr\΢͸g^Ȱ׃rlF͜ؽ˼ywȼȰˮƽXȱѪr͜أ33棩BoPDBuȰˮƽ0.3mumol/Lߵ4.8mumol/Lw½oStaurosporine@pٹȰˮƽYXȱѪ^ywȼȰˮƽcضȳهPϵPKCаl]ҪStaurosporineͨ^PKCĻ԰l]ȱѪXoCƿc͜Xo[18]԰lԸѪ̕ǰXȱѪģ\øЧҺɫV^PKCƄȱѪTİጷŵӰlFpiȄ}]¹Ȱ@ӣքeȌ211916GABAҲoH7ָ˷քe½յ334HA1004ȰGABAӰ^СጷܱHA1004^H7Сa@Nd^Ժ԰ጷřCƲͬ[19]Y@ʾȱѪXprPKCጷҪ{ܾͬøخPKCƄ߀ܜppȱѪXˮ[ڽYɳ΂i}]SDMCAƳɆ΂Xˮ[ģ^ɷNPKCƄH7ͿAXˮ[ӰlFߌɷNģXˮ[зPKCƄStaurosporineֱע뺣RCA1ίɳXȱѪlF@ȱѪ/ٹIJ׃pټ@NԪoЄهPϵ СY PKCͬøN^ǽz/Kᵰ׼øһͬøĽMֲYԼ_{CƲͬcwȶN^̵{P_lPKCͬøخ׿[ȷڼXѪܼίаl]Ҫ I 1 Mellor H,et al.Biocherm J,1998332:281 2 Nishizuka Y.FASEB J,19959:484 3 Hofmann J.FASEB J,199711:649 4 Flynn P,et al.J Biol Chem,1998273(5):2698 5 Wu SL,et al.J Biol Chem,1998273(5):1130 6 Yavin E,et al.J Neurochem,199565:2594 7 Onodera H,et al.Brain Res,1998481:1 8 Hara H,et al.Brain Res,1997774:69 9 Tohyama Y,et al.Brain Res,1997750:155 10 Harada K,et al.J Neurochem,199972(6):2556 11 Buchner K,et al.Mol Brain Res,199964(2):222 12 Islam N,et al.Indian J Physion Pharmacol,199539(1):37 13 Sieber FE,et al.Stroke,199829:1445 14 Savithiry S,et al.Mol Chem Neuropathol,199421:1 15 Miettinen S,et al.J Neurosci,199616(19):6236 16 Zablocka B,et al.Brain Res,1998779:254 17 Saluja I,et al.Neurochem Res,199924(5):669 18 Boris-Moller F,et al.Mol Chem Neuropathol,199835(1-3):133 19 Nakane H,et al.Brain Res,1998782:290
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